Table 2.
Helper | rep and cap | Copies of cap | Copies of rep | |
---|---|---|---|---|
AAV vector | plasmid | plasmids | per 106 vg | per 106 vg |
ACWRZn | pLadeno5 | pMTrep2, pCMVcap6 | 170 | 150 |
ARAP4 prep 1 | pLadeno5 | pMTrep2, pCMVcap6 | 58 | 70 |
ARAP4 prep 2 | pLadeno5 | pMTrep2, pCMVcap6 | 14 | 16 |
ARAP4 prep 3 | pLadeno5 | pMTrep2, pCMVcap6 | 36 | 32 |
ARAP4 prep 4 | pDGM6 | pDGM6 | 32 | 38 |
ACAGhAAT prep 1 | pDGM6 | pDGM6 | 26 | 27 |
ACAGhAAT prep 2 | pDGM6 | pDGM6 | 49 | 63 |
ACF3’B | pDGM6 | pDGM6 | 7.0 | 9.1 |
Virus was prepared for qPCR by treating 1010 to 1011 genome-containing particles (determined by Southern analysis) with benzonase. Next virion DNA was extracted as described in Materials and Methods and was subjected to qPCR. Results are expressed as copies of DNA per 106 vector genomes as determined by Southern analysis. For the ACWRZn vector, the genome number was checked by qPCR after benzonase treatment and purification of virions, and was 63% of the value determined by Southern analysis. Results are means of two experiments done in triplicate.