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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Gene Ther. 2010 Dec 16;18(4):411–417. doi: 10.1038/gt.2010.167

Table 2.

Quantitation of rep and cap DNA in AAV vector virions by qPCR a

Helper rep and cap Copies of cap Copies of rep
AAV vector plasmid plasmids per 106 vg per 106 vg
ACWRZn pLadeno5 pMTrep2, pCMVcap6 170 150
ARAP4 prep 1 pLadeno5 pMTrep2, pCMVcap6 58 70
ARAP4 prep 2 pLadeno5 pMTrep2, pCMVcap6 14 16
ARAP4 prep 3 pLadeno5 pMTrep2, pCMVcap6 36 32
ARAP4 prep 4 pDGM6 pDGM6 32 38
ACAGhAAT prep 1 pDGM6 pDGM6 26 27
ACAGhAAT prep 2 pDGM6 pDGM6 49 63
ACF3’B pDGM6 pDGM6 7.0 9.1
a

Virus was prepared for qPCR by treating 1010 to 1011 genome-containing particles (determined by Southern analysis) with benzonase. Next virion DNA was extracted as described in Materials and Methods and was subjected to qPCR. Results are expressed as copies of DNA per 106 vector genomes as determined by Southern analysis. For the ACWRZn vector, the genome number was checked by qPCR after benzonase treatment and purification of virions, and was 63% of the value determined by Southern analysis. Results are means of two experiments done in triplicate.