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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Gene Ther. 2010 Dec 16;18(4):411–417. doi: 10.1038/gt.2010.167

Table 5.

Quantitation of AAV virions containing rep and cap sequences in AAV vector preparations a

Copies of cap Copies of rep
AAV Vector cap plasmid per 106 vg per 106 vg
ACZn CMVcap6 240 240
Captron 54 110
ACF3’B CMVcap6 4.1 9.5
Captron 9.8 15
ACMVcFIX CMVcap6 140 150
Captron 55 110
a

Virion DNA was prepared for qPCR as described for Table 2 and in Materials and Methods. Vector genomes were quantitated by Southern analysis for the ACF3’B vector and by qPCR for the ACZn and ACMVcFIX vectors by using primers for βgal or cFIX, respectively. Results are means of two experiments done in triplicate. Results for ACMVcFIX are means of experiments using two different vector preparations.