Table 5.
Copies of cap | Copies of rep | ||
---|---|---|---|
AAV Vector | cap plasmid | per 106 vg | per 106 vg |
ACZn | CMVcap6 | 240 | 240 |
Captron | 54 | 110 | |
ACF3’B | CMVcap6 | 4.1 | 9.5 |
Captron | 9.8 | 15 | |
ACMVcFIX | CMVcap6 | 140 | 150 |
Captron | 55 | 110 |
Virion DNA was prepared for qPCR as described for Table 2 and in Materials and Methods. Vector genomes were quantitated by Southern analysis for the ACF3’B vector and by qPCR for the ACZn and ACMVcFIX vectors by using primers for βgal or cFIX, respectively. Results are means of two experiments done in triplicate. Results for ACMVcFIX are means of experiments using two different vector preparations.