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. Author manuscript; available in PMC: 2011 Apr 8.
Published in final edited form as: Oncogene. 2000 Nov 2;19(46):5270–5280. doi: 10.1038/sj.onc.1203906

Figure 9.

Figure 9

HPV-18 E6-induced degradation of MAGI-1 in vitro. In vitro-translated FLAG epitope-tagged MAGI-1c (MAGI-1c) was mixed with in vitro-translated wild-type 18E6, mutant 18E6-V158A, wild-type 16E6, mutant 16E6-T149D/L151A, or control water-primed lysates and incubated at 30°C for the indicated times. At each time point, reactions were immunoprecipitated with FLAG antibodies, and recovered proteins were separated by SDS–PAGE and visualized by autoradiography (left panel). The amount of in vitro-translated E6 protein used in each assay is shown (right panel)