Table 1. Quantification of FiGs in wild-type and gene-disruptant parasites.
WT* | WT** | PfCCp1-KO* | Pfs48/45-KO** | Pfs230-KO** | ||
---|---|---|---|---|---|---|
Culture 1 | Cells with FiGs (%) | 54.0 ± 2.00 | 46.0 ± 4.00 | 34.0 ± 3.46 | 36.0 ± 3.46 | 30.7 ± 3.06 |
No. of FiGs per cell (range) | 2.7 (1-7) | 3.3 (1-8) | 2.3 (1-6) | 2.7 (1-11) | 3.0 (1-7) | |
Length (μm; range) | 19.3 (2-120) | 13.7 (2-140) | 13.3 (2-90) | 17.3 (2-140) | 21.7 (2-180) | |
Culture 2 | Cells with FiGs (%) | 55.3 ± 7.57 | 32.7 ± 2.31 | 22.0 ± 2.00 | ||
No. of FiGs per cell (range) | 2.0 (1-6) | 2.0 (1-10) | 2.3 (1-8) | |||
Length (μm; range) | 18.0 (2-120) | 18.0 (2-120) | 16.7 (2-140) |
Detected by immunolabeling of Pfs230 (*)
Detected by immunolabeling of Pfs25 (**);
for each condition 50 activated gametocytes were counted in triplicate.