Fig. 1.

PKR and eIF2α are phosphorylated during RSV infection. (A) HEp-2 cells were infected with RSV (MOI = 1.0 pfu/cell) for the indicated times, fixed, and processed for immunofluorescence. Anti-RSV P monoclonal antibody was used to localize viral protein and appears green in the merge panel. Anti-TIA-1 antibody was used to detect stress granule formation and appears red in the merge panel. Phosphorylated eIF2α (peIF2α) appears blue in the merge panel. (B) HEp-2 cells were mock-inoculated or inoculated with RSV (MOI = 1.0 pfu/cell) for 24 h. Cell lysates were analyzed by immunoblots for GAPDH, RSV P, phosphorylated PKR, or total PKR. Relative protein densities comparing RSV-infected cells to mock-infected cells were quantified using Li-Cor Odyssey imaging software.