Fig. 6.

Treatment with 2-AP inhibits RSV replication independent of PKR. (A) Wild-type HEp-2 cells or HEp-2 cells transduced with non-targeting shRNA (NonT) or PKR-specific shRNA were pretreated with 10 mM 2-AP or vehicle. Cells were inoculated with RSV (MOI = 0.1 pfu/cell) in the presence of 2-AP or vehicle for the indicated intervals. Titers of cell-associated virus were determined for each time point by plaque assay. (B) Each cell type from (A) was pretreated with 10 mM 2-AP or vehicle and inoculated with RSV (MOI = 1.0 pfu/cell) for 24 h in the presence of 2-AP or vehicle. Cell lysates were subjected to immunoblotting for RSV P protein and GAPDH. The relative amount of RSV P protein was quantified for each cell type and compared to that in wild-type cells treated with vehicle. Error bars indicate standard deviations.