Table 1.
Temperature | Treatment | Without competition | With competition | ||||
---|---|---|---|---|---|---|---|
μ [h−1] | Yield | p value | μ [h−1] | Yield | p value | ||
25°C | Control | 0.076 | 2.30 × 104 | – | 0.002 | 0.04 × 104 | – |
50 clad | 0.120 | 6.31 × 104 | <0.01 | 0.068 | 2.99 × 104 | <0.01 | |
150 clad | 0.176 | 21.3 × 104 | <0.01 | 0.166 | 14.9 × 104 | <0.01 | |
23°C | control | 0.095 | 4.39 × 104 | – | 0.025 | 0.82 × 104 | – |
50 clad | 0.184 | 20.0 × 104 | <0.01 | 0.088 | 11.3 × 104 | <0.01 | |
150 clad | 0.188 | 61.0 × 104 | <0.01 | 0.095 | 10.3 × 104 | <0.01 | |
20°C | Control | 0.128 | 10.8 × 104 | – | 0.106 | 13.8 × 104 | – |
50 clad | 0.134 | 69.8 × 104 | <0.01 | 0.137 | 105 × 104 | <0.01 | |
150 clad | 0.130 | 61.0 × 104 | <0.01 | 0.158 | 39.9 × 104 | <0.01 |
Data indicate calculated growth rates (μ) and growth yield of V. cholerae non-O1/non-O139 without (control) and with (50 and 150) added cladocerans (clad)
All experiments were performed ‘with competition’ (2-μm filtered lake water) and ‘without initial competition’ (sterile filtered lake water) by the natural bacterial community
Each single μ and yield value is derived from one growth curve consisting of 12 consecutive sampling points
The p value indicates the significance of the difference in cell numbers between control and the cultures with cladocerans (paired T test; based on cell number data in logarithmic and stationary phase)