Figure 3.

PPP1R12A and the p85 regulatory subunit of PI 3-K interact with IRS-1. L6 myotubes were serum-starved for 4 h and either left untreated or stimulated with insulin (100 nM) for 15 min at 37 °C. One mg of lysate proteins were immunoprecipitated with normal IgG or IRS-1 antibodies and Western blotted with antibodies specific to either IRS-1, PPP1R12A, or p85; each graph is representative of data obtained from three independent experiments