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. 2011 Apr 6;100(7):1708–1717. doi: 10.1016/j.bpj.2011.02.039

Figure 1.

Figure 1

Representative time course of the frequency shift upon stepwise ezrin wt addition (a–f) to a solid-supported (A) OT-DOPC/DOGS-NTA-Ni (9:1) or (B) OT-POPC/PIP2 (9:1) membrane in 20 mM Tris/HCl, 50 mM KCl, 0.1 mM EDTA, pH = 7.4. (g) Rinsing with pure buffer. Concentrations in panel A: (a) 13 nM, (b) 39 nM, (c) 90 nM, (d) 161 nM, (e) 249 nM, and (f) 280 nM. Concentrations in panel B: (a) 19 nM, (b) 44 nM, (c) 91 nM, (d) 148 nM, (e) 233 nM, and (f) 282 nM.