Table 2.
Parameters obtained from QCM-measurements on three different ezrin species bound to DOGS-NTA-Ni or PIP2-containing membranes
| Membrane protein | DOGS-NTA-Ni |
PIP2 |
||||
|---|---|---|---|---|---|---|
| T567A | wt | T567D | T567A | wt | T567D | |
| Δfmax/Hz | 58 ± 13(n = 5) | 67 ± 19(n = 3) | 64 ± 5(n = 5) | 66 ± 4(n = 6) | 46 ± 8(n = 3) | 49 ± 5(n = 6) |
| Reversibility/% | 9 ± 7(n = 6) | 7 ± 5(n = 7) | 8 ± 2(n = 6) | 16 ± 10(n = 5) | 19 ± 11(n = 4) | 14 ± 7(n = 10) |
| KD/nM | 47 ± 6(n = 5) | 14 ± 2(n = 3) | 21 ± 5(n = 5) | 45 ± 12(n = 6) | 52 ± 32(n = 3) | 18 ± 9(n = 6) |
Values are given as mean values ± SD. The term n gives the number of independent experiments.