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. 2011 Apr 6;100(7):1737–1746. doi: 10.1016/j.bpj.2011.02.028

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© 2011 by the Biophysical Society.

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Inter-thick filament spacing (A) and intensity ratio, or I_2,0/I_1,0 (C) of IFM from resting, live flies and relaxed fibers compressed with dextran T-500 (% w/v) for RLC mutants and control. Note that some symbols are hidden (e.g., control at 2% dextran) behind overlapping measurements. Bulk modulus (B), or compressive elastic modulus, calculated at 4% dextran T-500 from the relationship between inter-thick filament spacing and osmotic compression for relaxed fibers. Within dextran concentration: asterisk (^∗) indicates a significant difference from control; pound sign (#) indicates a significant difference from Δ2-46 and S66A,S67A; and Ext and Phos indicate significant differences between N-terminal extension length (normal versus truncated) and phosphorylation sites state (normal versus disrupted), respectively. Fibers examined: Δ2-46 = 15; S66A,S67A = 15; Δ2-46; S66A,S67A = 10; and control = 12.