Skip to main content
. 2011 Apr 6;100(7):1819–1828. doi: 10.1016/j.bpj.2011.01.033

Figure 5.

Figure 5

Cell-cell interactions imaged by SPRIE and validated by CLSM. (A) Cell-cell interactions of HUVECs imaged by SPRIE. (B and C) Intensity topography of inserted dotted rectangles in A. The arrows indicate fibril-like cell-cell connections. (D) CLSM image for VE-cadherin (red), actin (green), and nuclei (blue) on the same cells shown in A. (E) Histogram of the fluorescence intensity and SPRIE signal of line E inserted in A and D. The green line indicates actin, and the red line indicates VE-cadherin. The black line indicates SPRIE signal. Note that VE-cadherin and actin overlap, and the length of the peak indicates the thickness of the fibril-like cell-cell connections. Scale bar = 50 μm.