Table 1.
Anchor primers | Arbitrary primers |
T12MG (10 μmol/L) | AP-10 (2 μmol/L), 5’-TAGCAAGTGC-3’ |
T12MA (10 μmol/L) | AP-11 (2 μmol/L), 5’-CAGACCGTTC-3’ |
T12MT (10 μmol/L) | AP-12 (2 μmol/L), 5’-TGCTGACCTG-3’ |
T12MC (10 μmol/L) | AP-14 (2 μmol/L), 5’-AATGGGCTGA-3’ |
M represents a degenerated mixture of dA, dG and dC. The 16 different primer sets used for PCR amplification were randomly combined from the four arbitrary primers and the four anchor primers.