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. 2011 Apr 6;100(7):1829–1836. doi: 10.1016/j.bpj.2011.02.024

Figure 4.

Figure 4

Comparison of EGFP molecular flow through a change in chromatin density (high-to-low or low-to-high) in a mitotic versus interphase nucleus upon ATP depletion. (A) Overlay of free EGFP and H2B-mCherry localization in the plane of the 3.3 μm line drawn in a mitotic nucleus under ATP-depleted conditions. (B) Intensity profile of the H2B-mCherry stain across the line measured in a mitotic nucleus under ATP-depleted conditions. (C) The pCF(8) carpet derived for intranuclear diffusion between adjacent chromatin density environments in a mitotic nucleus under ATP-depleted conditions. The red and green arrows indicate positions where diffusion through a change in chromatin density environment is tested. (D) Overlay of free EGFP and H2B-mCherry localization in the plane of the 3.3 μm line drawn in an interphase nucleus under ATP-depleted conditions. (E) Intensity profile of the H2B-mCherry stain across the line measured in an interphase nucleus under ATP-depleted conditions. (F) The pCF(9) carpet derived for intranuclear diffusion between adjacent chromatin density environments in an interphase nucleus under ATP-depleted conditions. The orange and cyan arrows indicate positions where diffusion through a change in chromatin density environment is tested. (G) A plot of the cross correlation function derived from the pCF(8) carpet in panel C for delayed diffusion through a change in chromatin density from the marked arrow positions: in (column 8 in red) and out (column 20 in green) of the chromosome of a mitotic nucleus under ATP-depleted conditions. (H) A plot of a cross correlation function derived from the pCF(9) carpet in panel F for delayed diffusion through a change in chromatin density from the marked arrow positions: in (column 3 in orange) and out (column 19 in cyan) of a high density chromatin bundle under ATP-depleted conditions.