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. 2011 Apr 6;100(7):1829–1836. doi: 10.1016/j.bpj.2011.02.024

Figure 5.

Figure 5

Comparison of EGFP molecular flow through a change in chromatin density (high-to-low or low-to-high) in a mitotic-versus-interphase nucleus in the C. elegans germ line. (A) The adult germ line of C. elegans expressing monomeric EGFP, with the nuclei stained with Hoechst 33342. (B) Overlay of free EGFP and Hoechst 33342 localization in the plane of the 3.3 μm line drawn in a nucleus actively undergoing mitosis in the mitotic zone. (C) Overlay of free EGFP and Hoechst 33342 localization in the plane of the 3.3 μm line drawn in an interphase nucleus in the mitotic zone. (D) Intensity profile of the Hoechst 33342 stain across the line measured in the nucleus actively undergoing mitosis. (E) Intensity profile of the Hoechst 33342 stain across the line measured in the interphase nucleus. (F) The pCF(9) carpet derived for intranuclear diffusion between different adjacent density chromatin environments in a nucleus actively undergoing mitosis. (G) The pCF(8) carpet derived for intranuclear diffusion between different adjacent density chromatin environments in the interphase nucleus.