Table 1.
Summary of real-time RT-PCR oligonucleotide primers used in measuring mRNA expression in mitochondrial structural genes, ETC-encoded genes, peroxiredoxins and neuroprotective genes of N2a cells treated with Aβ and MTAs, and N2a cells treated with MTA followed by incubation with Aβ.
| Gene | DNA Sequence (5′-3′) | PCR Product Size |
|---|---|---|
| Mitochondrial Structural Genes | ||
| Drp1 | Forward Primer GCGCTGATCCCGCGTCAT | 54 |
| Reverse Primer CCGCACCCACTGTGTTGA | ||
| Fis1 | Forward Primer GCCCCTGCTACTGGACCAT | 62 |
| Reverse Primer CCCTGAAAGCCTCACACTAAGG | ||
| MFN1 | Forward Primer TCTCCAAGCCCAACATCTTCA | 62 |
| Reverse Primer ACTCCGGCTCCGAAGCA | ||
| MFN2 | Forward Primer ACAGCCTCAGCCGACAGCAT | 56 |
| Reverse Primer TGCCGAAGGAGCAGACCTT | ||
| Opa1 | Forward Primer TGGGCTGCAGAGGATGGT | 59 |
| Reverse Primer CCTGATGTCACGGTGTTGATG | ||
| TOMM40 | Forward Primer CCTGCCCTCTGACCTTTCC | 60 |
| Reverse Primer GAGAGCTGGCAGCCAACAC | ||
| Cyclophilin D | Forward Primer CAGCCAAGCCCTCCAACTC | 58 |
| Reverse Primer GCCGATGTCCACGTCAAAG | ||
| BCL2 | Forward Primer TGAGACTCCCAGCATGAAGGA | 60 |
| Reverse Primer GCCTTCCACGCACTCCAT | ||
| HSP60 | Forward Primer GAAATGCTTCGACTACCCACAGT | 57 |
| Reverse Primer CCAGTGCCCGGGACACT | ||
| Mitochondrial-encoded Electron Transport Chain Genes | ||
| NADH (sub1) | Forward Primer CGGGCCCCCTTCGAC | 72 |
| Reverse Primer GGCCGGCTGCGTATTCT | ||
| NADH (sub3) | Forward Primer TGTACTCAGAAAAAGCAAATCCATATG | 75 |
| Reverse Primer AATAATAGAAATGTAATTGCTACCAAGAAAAA | ||
| NADH (sub5) | Forward Primer CGGACGAACAGAACGCAAATA | 70 |
| Reverse Primer TAAAATGAATCCGATGTCTCCGA | ||
| CYT B | Forward Primer TTATCGCGGCCCTAGCAA | 75 |
| Reverse Primer TAATCCTGTTGGGTTGTTTGATCC | ||
| COX 1 | Forward Primer GAAGAGACAGTGTTTCATGTGGTGT | 73 |
| Reverse Primer TCCTGGGCCTTTCAGGAATA | ||
| COX3 | Forward Primer CAGGATTCTTCTGAGCGTTCTATCA | 75 |
| Reverse Primer AATTCCTGTTGGAGGTCAGCA | ||
| ATP6 | Forward Primer TGTGGAAGGAAGTGGGCAA | 72 |
| Reverse Primer CCACTATGAGCTGGAGCCGT | ||
| Cytoprotective Antioxidant Enzyme Genes | ||
| Prx1 | Forward Primer TGGCTCGACCCTGCTGATAG | 61 |
| Reverse Primer GGAGCAGGATACCCAATTTTTG | ||
| Prx2 | Forward Primer CCCCTGAATATCCCTCTGCTT | 57 |
| Reverse Primer CGCCGTAATTCTGGGACAA | ||
| Prx3 | Forward Primer GGCCCCATTTCTTGGAT | 60 |
| Reverse Primer CAGGGCAGGCTAAGGGAAAG | ||
| Prx4 | Forward Primer CCTGTTGCGGACCGAATCT | 55 |
| Reverse Primer GGGTCCGGAACCGTTCAT | ||
| Prx5 | Forward Primer CCCGATCAAGGTGGGAGAT | 56 |
| Reverse Primer CCCGGTTCCCCTTCAAATA | ||
| Prx6 | Forward Primer TCTGGCAAAAAATACCTCCGTTA | 58 |
| Reverse Primer GCCCCAATTTCCGCAAAG | ||
| Neuroprotective Genes | ||
| FOXO1 | Forward Primer CCCGTCCTAGGCACGAACT | 56 |
| Reverse Primer ACGCGCCCAGAACTTAACTTC | ||
| PGC1α | Forward Primer GGACAGTCTCCCCGTGGAT | 57 |
| Reverse Primer TCCATCTGTCAGTGCATCAAATG | ||
| NMDA receptor | Forward Primer GGTCAGTTCTGTCCTGCACATC | 62 |
| Reverse Primer TGACTCTCCCGCGGAAAC | ||
| Housekeeping Genes | ||
| Beta Actin | Forward Primer ACGGCCAGGTCATCACTATTC | 65 |
| Reverse Primer AGGAAGGCTGGAAAAGAGCC | ||
| GAPDH | Forward Primer TTCCCGTTCAGCTCTGGG | 59 |
| Reverse Primer CCCTGCATCCACTGGTGC | ||