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. Author manuscript; available in PMC: 2011 Apr 8.
Published in final edited form as: Biochim Biophys Acta. 2005 Sep 26;1717(2):67–88. doi: 10.1016/j.bbamem.2005.09.010

Table 1.

E. coli MdfA confers a capacity for Na+- and K+-dependent pH homeostasis during an alkaline shift in the external pHa

Cytoplasmic pH after a gradual shift in pHout
pH 7.0→8.3 pH 7.0→9.3
CholineCl NaCl KCl CholineCl NaCl KCl
E. coli ΔmdfA mutant, control 7.9 7.8 7.8 9.3 9.3 9.3
E. coli ΔmdfA mutant, mdfA expressed from plasmid 7.6 7.6 7.6 9.3 7.9 7.8
a

Cytoplasmic pH was measured by the methylamine accumulation assay after cells were shifted from a growth medium at pH 7.0 to growth media at the final pH values indicated and had established logarithmic growth [122].