CD133+ prostate cancer cells express the AR. Dual-variable flow cytometry of CD133 (AC141) and AR in the LAPC-4, LNCaP, and CWR22Rv1 prostate cancer cell lines. Top, control staining in LAPC-4 using IgG antibodies (top left, CD133 versus IgG; top right, IgG versus AR). Similar controls were documented in the LNCaP and CWR22Rv1 cell lines. Bottom, dual labeling for CD133 and AR in LAPC-4, LNCaP, and CWR22Rv1 prostate cancer cells showing that CD133+ prostate cancer cells also express AR.