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. 2011 Apr 7;3(2):e00054. doi: 10.1042/AN20110006

Figure 2. Effect of methanol on GD2, SSEA-1 and GD3 immunoreactivity in human NSCs.

Figure 2

Human NSCs treated with or without methanol (MeOH) were immunostained with antibodies against GD2 (A), SSEA-1 (B) and GD3 (C). Human NSCs were positive for GD2 (green; A) and relatively weakly positive for GD3 (green; C). No immunoreactivity of human NSCs to antibodies against GD2 (A) and GD3 (C) after treatment with 100% methanol for 10 min to wash out lipids of plasma membranes indicates that the immunoreactivity is attributed to GD2 and GD3 gangliosides, but not to glycoproteins or proteoglycans. (B) Human NSCs treated with methanol were still positive for SSEA-1 (green), indicating that SSEA-1 is mainly carried by glycoproteins and/or proteoglycans in these cells. (D) Mouse embryonic NSCs prepared from mouse brains at embryonic day 14 in the form of neurospheres were stained with anti-GD3 antibody (green) as a positive control. Nuclei were stained with Hoechst 33258 (blue).