Fig. 3.

(A) Phosphoproteome-wide impact of each kinase and phosphatase. For all kinases and phosphatases, we computed the fraction of phosphopeptides affected relative to the total number of phosphopeptides affected by all kinases and phosphatases. The kinases and phosphatases were then ranked accordingly. Blue circles represent kinases, light blue circles represent essential kinases, and golden circles represent phosphatases. A large golden triangle indicates a strong growth or morphological phenotype of a given mutant, whereas a small blue triangle represents a weak growth or morphological phenotype of a given mutant. Right side: examples of kinases that showed either a low or a high effect on the phosphoproteome regions, together with their known cellular functions. (B) For each kinase and phosphatase, the biological processes enriched among their regulated phosphoproteins were computed. Each column corresponds to a biological process, whereas each row corresponds to a given kinase or phosphatase (kinases are depicted in blue, essential kinases in light blue, and phosphatases in gold). The color scale denotes the statistical significance of the observed enrichment. Magnified inset: an example for three clustered kinases, for which a related set of processes is observed enriched among their substrates.