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CD4+ T-cell numbers in the colonic lamina propria of RAG−/− mice reconstituted with naïve T-cells. CD4+-cell numbers were obtained by multiplying the total number of viable cells isolated from the colonic lamina propria by the percentage of total cells positive for CD4 as determined by flow cytometry. Data represent means ± SEM for n = 3–4 mice in each group. Comparisons between groups were determined by one-way ANOVA.
