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. Author manuscript; available in PMC: 2011 Apr 8.
Published in final edited form as: Exp Hematol. 2009 May 15;37(8):909–923. doi: 10.1016/j.exphem.2009.05.002

Figure 4.

Figure 4

DL1 ligand and cytokines are both necessary for differentiation of EML cells into double-negative (DN) T-cell precursors. Differentiation of EML cells into DN2 and DN3 cells was examined in (A) day 7 and day 12 EML/OP9 stromal cells expressing Notch ligand Delta-like 1 (OP9-DL1) cocultures supplemented with all cytokines (stem cell factor [SCF], interleukin-7 [IL-7], and Fms-like tyrosine kinase 3 ligand [Flt3L]) or with SCF alone, (B) day 7 and 12 EML/OP9 cocultures supplemented with all cytokines or SCF alone, and (C) day 7 and 12 EML cell cultures with all cytokines in the absence of OP9 stromal cells. The numbers in each quadrant indicate the percentage of cells expressing the CD44 and/or CD25 marker.