Figure 7.

Antioxidant restoration of E47-deficient MPPs. (A) Quantitative PCR analysis of redox-associated genes in purified HSCs (flk2⁻CD150⁺CD48⁻ LSKs) or MPPs (flk2⁺ LSKs) from E47 KO mice compared with E47 WT mice. Data are mean ± SD of triplicate wells, representative of 2 or 3 independent sorts. *P < .05. (B) Mice treated with 1 mg/mL N-acetyl cysteine in the drinking water were killed after 14 days, and lymphoid organs stained to detect bone marrow multipotent progenitors (flk2⁻ and flk2⁺ LSKs), common lymphoid progenitors (CLPs: lin⁻IL7R⁺AA4.1⁺Sca-l^lo), and early thymic progenitors precursors (ETPs: lin⁻CD44⁺CD25⁺c-kit⁺IL-7R⁻). (C) Quantitation of hematopoietic subsets in B (n = 5 mice/group). *P < .05. ns indicates not significant.