Skip to main content
. 2011 Mar 23;8:134. doi: 10.1186/1743-422X-8-134

Table 1.

Overview of ϕKMV-related bacteriophages included in this study, all infecting P. aeruginosa.

Phage Isolated in Genome (bp) # ORFs DTR length GC (%) ϕKMV identitya Accession number Kadsorption (ml/min)b Latent period (min) Burst size (PFU/cell) KAPS/PT5c KAPS/PNC101c
ϕKMV Moscow, Russia 42,519 52 414 62.3 100 NC_005045 1.6 × 10-8 28 116 259 849
LKD16 Leuven, Belgium 43,200 54 428 62.6 83 NC_009935 - 27 120 87.5 -
LUZ19 Leuven, Belgium 43,548 54 472 62.2 89 NC_010326 1.2 × 10-7 22 122 - -
ϕKF77 Moscow, Russia 43,152 53 454 62.8 90 NC_012418 1.5 × 10-8 23 129 56.2 -
PNM Tbilisi, Georgia 42,721 51 422 62.3 94 - ND ND ND - 23
PT2 Tbilisi, Georgia 42,961 54 488 62.0 97 NC_011107 9.2 × 10-9 21 116 737 51
PT5 Tbilisi, Georgia 42,954 52 413 62.0 95 NC_011105 7.3 × 10-9 21 134 598 679
PT6 Tbilisi, Georgia ND ND ND ND ND - 2.1 × 10-8 25 166 257 857
PNC101 Tbilisi, Georgia ND ND ND ND ND - ND ND ND 60 753
LUZ2 Leuven, Belgium ND ND ND ND ND - ND ND ND 270 -
LKA1 Leuven, Belgium 41,593 56 298 60.9 ND NC_009936 3.9 × 10-9 41 225 - -

a Identity with ϕKMV, expressed as % identical nucleotides according to the Stretcher algorithm (EMBOSS suite).

b The adsorption constant k was calculated from the unabsorbed phage at a multiplicity of infection of 1:1 by applying the formula ka = 2.3/Bt log P0/P, where P0 is initial number of phage, P is number of unabsorbed phage particles after time t and B is number of bacteria (4). ND, not determined.

c Serological neutralization assay using PT5 and PNC101 APS. Dilutions (1/50- 1/1000) of APS were mixed with 106 bacteriophage, samples were titered after different incubation times (5, 15 and 30 min) at 37°C. K, rate constant of neutralization, defined as k = 2.3 D/t logP0/P, where P0 - count of bacteriophage plaques at zero time, P - count of bacteriophage plaques after t time, D - dilution of APS.