Figure 2.

Gene expression analysis of CD8⁺ SP T cells obtained from HSCs cultured on OP9-DL1 cells. Gene expression analysis by QRT-PCR from coculture-derived CD8 SP T cells (CD8⁺ CD4^- CD3⁺). CD8 SP, CD4 SP T cells and CD33⁺ myeloid cells were purified from the Lin⁺ fraction of UCB samples and served as controls. Transcript levels for the indicated genes were normalized to human β-actin, and these data are representative of 3 independent experiments, with the standard error bars shown corresponding to values obtained from triplicate wells within an individual experiment.