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Determination of NPM2 mRNA abundance in oocytes from growing and persistent dominant follicles by quantitative real time PCR analysis (mean ± SEM, n = 4). Bovine HIST2H2AA4 gene was used as an endogenous control for data normalization. Relative abundance of NPM2 mRNA is significantly lower in oocytes from persistent than from growing follicles. Different letters indicate statistical difference (P < 0.05).
