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. 2011 Mar 27;11:108. doi: 10.1186/1471-2407-11-108

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Copyright ©2011 Escrevente et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of neuraminidase and sugars on SKOV3 exosomes uptake. (A) Exos-CFSE (20 μg protein) were incubated with 15 mU V. cholerae neuraminidase or corresponding buffer for 2 h before Exos-CFSE uptake. (B) Effect of 150 mM D-glucose (Glc), α-L-fucose (Fuc), α-D-mannose (Man), β-lactose (Lac), D-galactose (Gal) and D-N-acetylglucosamine (GlcNAc) (30 min pre-incubation) on Exos-CFSE uptake (4 h) monitored by flow cytometry analysis. Uptake efficiency was calculated relatively to the uptake without treatment, considered as 100%. Results are displayed in relative percentages ± S.D. The results shown are representative of three independent experiments performed in duplicate.