Table 4. Recommended fiber combinations.
Suggested SPME Fiber Combination | Percent coveragea | ||
GC-MS(Identities) | GC-MS(Retention Time) | GC-FID(Retention Time) | |
50/30 µm CAR-DVB-PDMS85 µm CAR-PDMS65 µm DVB-PDMS7 µm PDMS60 µm PEG75 µm CAR-PDMS85 µm PA | 99 | 97 | 99 |
50/30 µm CAR-DVB-PDMS85 µm CAR-PDMS65 µm DVB-PDMS7 µm PDMS60 µm PEG75 µm CAR-PDMSb | 93 | 93 | 97 |
50/30 µm CAR-DVB-PDMS85 µm CAR-PDMS65 µm DVB-PDMS7 µm PDMS60 µm PEG | 87 | 88 | 94 |
50/30 µm CAR-DVB-PDMS85 µm CAR-PDMS65 µm DVB-PDMS7 µm PDMS | 84 | 81 | 91 |
50/30 µm CAR-DVB-PDMS85 µm CAR-PDMS65 µm DVB-PDMS | 78 | 71 | 86 |
percentage of total metabolites obtained using all eight fibers.
substitution with the 85 µm PA fiber results in 92, 92, and 97% coverage, respectively.