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. 2011 Apr 8;6(4):e18674. doi: 10.1371/journal.pone.0018674

Figure 3. Recombinant SFRP3 decreases migration and invasion of melanoma cells.

Figure 3

A, Invasion assay using Matrigel invasion chambers. A2058 cells were resuspended in serum free media and treated in the upper chamber with 1 µg/ml of rSFRP3 or carrier (0.1% BSA in PBS) and allowed to invade for 24 h. The error bars represent SEM, (n = 5). B, Wound healing assay. A2058 cells were treated with either 1 µg/ml of rSFRP3 (dashed line, squares) or carrier (full line, circles). Pictures were taken of the scratches at 0, 16, 24 and 48 h. Data is given as percentage of wound area closed at each time point. The error bars represent SEM, (n = 5). C, Migration assay, A2058 cells were treated with either 1 µg/ml of rSFRP3 or carrier in serum-free media and migration of non-dividing individual cells was analyzed manually by tracking the speed of cells for 12 h. The error bars represent SEM, (n = 5). * = p<0.05. D, Wnt5a protein expression analysis. Wnt5a expression was analyzed in A2058 cells and HTB63 cells using western blotting.