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. 2011 Apr 8;6(4):e18674. doi: 10.1371/journal.pone.0018674

Figure 5. SFRP3 inhibits migration in melanoma cells by antagonizing Wnt5a.

Figure 5

A, Migration assay using Wnt5a knockdown cells. siRNA transfected A2058 cells were grown in serum-free media for 24 h and then treated with 1 µg/ml rSFRP3 or carrier. Migration was analyzed using time-lapse microscopy for 24 h. The error bars represent SD, (n = 4). B, Wnt5a knockdown. The knockdown of Wnt5a in A2058 cells were analyzed using western blot but also by RT-PCR (Figure S4). C, Migration assay using rWnt5a and rSFRP3 in A2058 cells. Cells were pre-treated with either carrier or 1 µg/ml rSFRP3 in serum-free media for 24 h. The cells were then treated with rWnt5a 0.2 µg/ml and analyzed using time-lapse microscopy as in A for 24 h. The error bars represent SD, (n = 4). D, SFRP3 conditioned medium and migration assay. HTB63 cells were transfected with pCMV6-Empty-Vector or pCMV6-SFRP3 and kept in serum-free media for 24 h. These media were transferred to non-transfected HTB63 cells that were then analyzed using time-lapse microscopy as in A. Error bars represent SD (n = 3). * = p<0.05.