Table 2. Purification of recombinant xylanase from E. coli harboring pHsh-ex-xynA2.
Steps | Total protein(mg) | Total activity(U) | Specific activity (U/mg) |
Periplasmic fraction | 29.4 | 20609 | 701 |
Heat treatment | 21.2 | 17214 | 812 |
DEAE Sepharose FF | 3.1 | 4644 | 1498 |