. Author manuscript; available in PMC: 2012 Apr 30.

Published in final edited form as: Mol Cell Endocrinol. 2011 Jan 26;337(1-2):16–23. doi: 10.1016/j.mce.2011.01.016

Table 1.

Binding parameters, maximal Buserelin-stimulated inositol phosphate (IP) production, and ED₅₀ of the WT and mutant hGnRHRs (means ± SEM from three or more independent experiments)

GnRHR	IC₅₀ (nM)	Cell surface receptor number (fmol × 10⁵ cells)	Max IP production (% GnRHR WT)	ED₅₀ (pM)
WT	2.7 ± 0.2 ^a	4.6 ± 0.9 ^a	100 ^a	22.1 ± 5.4 ^a
T104I	-	-	25 ± 3 ^b	-
Y108C	-	-	3 ± 0.5 ^c	-

WT ΔK191	2.2 ± 0.2 ^a	11.5 ± 2.2 ^b	258 ± 8 ^d	13.5 ± 3.4 ^a
T104I ΔK191	3.4 ± 0.6 ^a,b	3.8 ± 0.6 ^a,c	166 ± 4 ^e	24.0 ± 3.1 ^a
Y108C ΔK191	6.7 ± 0.9 ^b	4.4 ± 0.6 ^a	73 ± 9 ^f	76 ± 10 ^b

WT/C-tail	2.5 ± 0.2 ^a	16.0 ± 1.4 ^b	277 ± 5 ^d	13.5 ± 1.4 ^a
T104I/C-tail	4.3 ± 0.9 ^a,b	2.1 ±0.6 ^a	167 ± 8 ^e	72.1 ± 8.0 ^b
Y108C/C-tail	-	-	13 ± 3 ^g	-

Different superscript letters indicate statistically significant differences between means in the same column (p<0.05 for IC₅₀ and cell surface receptor number; p≤ 0.01 for maximal IP production; p<0.01 for ED₅₀).