FIG. 2.

Schematic representation of the expansion of the 293 cell train used for the 50-liter rVSV GMP production lot. Cells from the 293 master cell bank were thawed and expanded for the manufacture of VSV. Cells were passaged twice per week with trypsin. An additional 10-layer Cell Factory (CF) was seeded for each week's infection in order to be able to determine the number of cells in each CF (not indicated). Additional flasks (2 × T-175) were also seeded as part of the parallel culture for each week's manufacturing production (not indicated). Color images available online at www.liebertonline.com/hum.