Table 2.
Sample ID | Volume | Infectious titer (PFU/ml) | Total infectious units (PFU) |
---|---|---|---|
Clarified supernatant | 9 liters | 2.4 × 108 | 2.2 × 1012 |
Post-Benzonase | 9 liters | 6.0 × 108 | 5.4 × 1012 |
Elution peak (1.2M) | 312 ml | 1.7 × 108 | 5.3 × 1011 |
Post-UF/DF | 105 ml | 5.2 × 109 | 6.0 × 1011 |
Formulated bulk | 116 ml | 7.2 × 109 | 8.4 × 1011 |
Filtered (0.2 μm) | 100 ml | 1.2 × 1010 | 1.3 × 1012 |
DF, diafiltration; UF, ultrafiltration.