Table 3.

Virus Yield for Clinical Lot In-Process Samples

Subbatch	Sample ID	Volume	Infectious titer (PFU/ml)	Total infectious units (PFU)
1	Clarified supernatant	9 liters	3.0 × 108	2.7 × 1012
	Post-Benzonase	9 liters	1.5 × 108	1.4 × 1012
	Elution peak (1.2M)	330 ml	1.6 × 109	0.5 × 1012
	Post-UF/DF	116 ml	2.4 × 1010	2.8 × 1012
2	Clarified supernatant	9 liters	3.3 × 108	2.7 × 1012
	Post-Benzonase	9 liters	1.5 × 108	1.4 × 1012
	Elution peak (1.2M)	306 ml	4.7 × 109	1.4 × 1012
	Post-UF/DF	111 ml	2.3 × 1010	2.6 × 1012
3	Clarified supernatant	9 liters	3.8 × 108	4.3 × 1012
	Post-Benzonase	9 liters	2.2 × 108	2.0 × 1012
	Elution peak (1.2M)	342 ml	4.9 × 109	1.7 × 1012
	Post-UF/DF	117 ml	2.8 × 1010	3.3 × 1012
4	Clarified supernatant	9 liters	2.0 × 108	1.8 × 1012
	Post-Benzonase	9 liters	ND	ND
	Elution peak (1.2M)	344 ml	ND	ND
	Post-UF/DF	117 ml	2.3 × 1010	2.6 × 1012
5	Clarified supernatant	9 liters	ND	ND
	Post-Benzonase	9 liters	ND	ND
	Elution peak (1.2M)	366 ml	ND	ND
	Post-UF/DF	125 ml	2.4 × 1010	2.7 × 1012

DF, diafiltration; ND, not determined; UF, ultrafiltration.