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Luciferase mRNA expression kinetics by transcriptionally targeted oncolytic Ads with luciferase reporter gene inserted into the late transcription unit. Keratinocytes ([A and B] HaCat) or melanoma cells ([C] SK-MEL-28) were infected with Ads at 10 TCID50 per cell in the presence or absence of AraC. Cells were harvested at indicated time points post-infection, and luciferase mRNA was quantified by qPCR. The results were reproduced in an independent experiment.
