Figure 3. BMS-754807 is active in basal-like/triple-negative breast cancer cell lines.

A. The concentration of BMS-754807 required to reduce growth by 50% (IC₅₀) was calculated for each cell line using monolayer proliferation and MTS assay. Breast cancer cells were seeded at 1,000 to 12,000 cells per well depending on the cell line in 96 well microtiter plates and incubated overnight. BMS-754807 was serially diluted and added. After 72 hr exposure, MTS assay was performed. Bars represent the average IC₅₀ (μM) of each breast cancer cell line. For seven cells lines the IC₅₀ was not reached (16.49μM and 25μM, respectively). Sensitive cell lines have an IC₅₀ below the mean of the group of cells (6.4μM); resistant cell lines are above the mean. The graph shows cell lines ranked according to its IC₅₀. Black bars represent cell lines that have a basal-like gene expression signature (TNBC) based upon the studies of Neve et al (38) and grey bars represent luminal or HER2 positive cell lines. B. IC₅₀ is plotted alongside the t-score for the IGF signature for each cell line. Pearson correlation shows there is a significant correlation (r=-0.41, p=0.014), with a higher t-score (indicating an active IGF-IR pathway) being associated with a greater response (lower IC₅₀) to BMS-754807.