Skip to main content
. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Clin Cancer Res. 2010 Dec 22;17(8):2314–2327. doi: 10.1158/1078-0432.CCR-10-1903

Figure 5. TNBC tumorgrafts express high levels of active IGF-IR.

Figure 5

A. Three different TNBC tumorgraft lines (2147, 2665A, MC1) were harvested and processed in paraffin. Immunohistochemistry was performed on five-micrometer sections for anti-pY-IGF-IR (Phospho-IGF-IR) and total IGF-IR. Microarray analysis was performed on these tumorgrafts. Based on the gene expression data an IGF signature t-score was calculated for each tumorgraft as indicated underneath the representative picture. B. The same three tumorgraft lines were lysed and analyzed by immunoblot and probed using IGF-IR specific antibodies as well as total/phospho-specific antibodies for IRS1, AKT, ERK1/2. β-actin was used as a loading control. Tumorgraft lines that were not relevant to this study were cropped out between 2665A and MC1.