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. Author manuscript; available in PMC: 2012 Mar 4.
Published in final edited form as: Cell Stem Cell. 2011 Mar 4;8(3):318–325. doi: 10.1016/j.stem.2011.01.016

Figure 2. Pre- and post-implantation-derived EpiSCs share gene expression and DNA methylation patterns that are distinct from other pluripotent states.

Figure 2

(A) Quantitative RT-PCR of pre-implantation-derived EpiSCs (E3.5) showed gene expression patterns consistent with post-implantation derived EpiSCs (E5.5) and not pre-implantation derived mouse ES cells (E3.5). (B) Correlations of whole genome microarray data showed a strong correlation between pre- and post-implantation derived EpiSCs and their clear distinction from other pluripotent cells such as FAB-SCs and mouse ES cells. Bisulfite pyrosequencing of pre-implantation-derived EpiSCs (E3.5) reveled hypermethylation of CpGs in the (C) Dppa3 promoter region and hypomethylation in (D) Oct3/4 promoter region. The location of analyzed CpGs is indicated in red.