Figure 4.
Y2 receptor binding is reduced in the locus ceruleus after Y2 gene deletion in the CEA. A–C, Pseudocolor-coded images of [125I]hPYY3-36 receptor autoradiographs of representative brain sections of conditional Y2 KO mice 4 weeks after rAAV-GFP (A) and rAAV-Cre (B) injections, respectively, and of a germ-line Y2 KO mouse (C), are shown. [125I]hPYY3-36 was almost abolished in germ-line Y2 KO mice, indicating the specificity of the ligand for Y2 receptors. Arrowheads denote the location of the locus ceruleus. Scale bar, 1 mm. In D, a quantitative evaluation of [125I]hPYY3-36 binding in the locus ceruleus is shown (n = 16). ***p < 0.001, one-way ANOVA, with Bonferroni post hoc test.