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. Author manuscript; available in PMC: 2011 Apr 11.
Published in final edited form as: Circ Cardiovasc Genet. 2010 Feb 2;3(2):138–146. doi: 10.1161/CIRCGENETICS.109.904698

Fig 5. Analysis of sarcomere gene exon-exon junctions by capillary electrophoresis of RTPCR products.

Fig 5

a. TNNT2 transcript and alternative splicing variant due to differential exon 7 5' splice acceptor use. b. Detection of variation of exon-exon junctions by capillary electrophoresis. A standard curve was generated by mixing known proportions of cloned wild-type and c.203_205del TNNT2. The peak area ratios from capillary electrophoresis analysis were linearly related to the input ratio. c. Representative chromatograms of control and ICM samples analyzed using the capillary electrophoresis assay. No significant difference in the proportion of c203_205del variant was detected between groups (n=15).