Table 2.
PCR primers used in this study
| Primer | Gene | Sequence (5′-3′) | Purpose |
| 395 | hph | CTC CAT ACA AGC CAA CCA CGG | deletion of derA |
| 396 | hph | CGT TGC AAG ACC TGC CTG AA | deletion of derA |
| 398 | hph/ble | CGC CAG GGT TTT CCC AGT CAC GAC AAG TGG AAA GGC TGG TGT GC | deletion of derA |
| 399 | hph | AGC GGA TAA CAA TTT CAC ACA GGA TCG CGT GGA GCC AAG AGC GG | deletion of derA |
| 579 | derA | CTG GTA TCT GCG GAT CTC TT | deletion of derA/probe1 |
| 580 | derA | GTC GTG ACT GGG AAA ACC CTG GCG GAC AGT GAG TGA GTG ATA TG | deletion of derA/probe1 |
| 581 | derA | TCC TGT GTG AAA TTG TTA TCC GCT TCC GAT GAC TTG CTG GCA TT | deletion of derA |
| 582 | derA | TCG AAC AGC TCT TTT CTG CC | deletion of derA |
| 584 | derA | GCG ACC GTG GCT ACT TCA GT | probe 2 |
| 585 | derA | GTT CAC ATT ATC CGT GTC TG | probe 2 |
| 586 | derA | GCC ACT TGA CAT AGA GCC CT | ΔderA complementation |
| 587 | derA | GAG TAT CCC AAA CAG CTG AC | ΔderA complementation |
| 408 | ble | TGC TCG CCG ATC TCG GTC AT | deletion of derA |
| 409 | ble | AGC GGA TAA CAA TTT CAC ACA GGA TTA AAG CCT TCG AGC GTC C | deletion of derA |
| 410 | ble | GAC AAG GTC GTT GCG TCA GTC | deletion of derA |
| 494 | bipA | GTC TGA TTG GAC GCA AGT TC | creation of bipA probe |
| 495 | bipA | ATC TGG GAA GAC AGA GTA CG | creation of bipA probe |
M13-derived sequences used for overlap PCR are underlined.