Figure 3.

Inhibition of HRV6 activation of human airway cells by silencing TLRs MDA-5 and RIG-I. Following RNA interference (receptor expression levels were reduced by 80% by RNA interference A) human airway cells were either not stimulated (grey bar charts), incubated with HRV6 (black bar charts), incubated with UV-inactivated HRV6 (white bar charts) and HRV6 ssRNA (stripped bar charts). The supernatants were harvested and assayed for IL6 cytokine secretion in 2 h (B) and IFNβ secretion in 4 h (C) using the Cytometric Bead Array (CBA) system (Becton Dickinson). Fluorescence was detected using a FACSC alibur (BectonDickinson). The data represents the mean of three independent experiments. Asterisks denote statistical significance (p < 0.001).