Table 2.
Oligonucleotide primers used in this study
| Primer designation | Genomic locationa | Primer Sequence (5′->3′)b | ORF | Orientation |
| gmhaF3b | 1082341-1082367 | TAC CCA AAT CGC TAA AGT AGG TGA GC | gmha | R |
| wlasaR3 | 1080361-1080392 | AAA CTG CAG CAC TTA GCC CAA ACC GAC CAG C | waaV | F |
| waaFR490bam2 | 1081397-1081317 | CGC GGA TCC GCG TTC CAA AGC TTG CAC CAG GGT TGA G | waaF | R |
| waaFF550bam2 | 1081458-1081482 | CGC GGA TCC GCG ATG ATA TTT TAA TCT TTG GTG CAG G | waaF | F |
| cj0984F339 | 918174-918196 | TAA TCT TTT TGG CAT TGT AAGG | Cj0984 | F |
| cj0987F913 | 920041-920019 | TTA GTA TTG ATA CAG ATT TTT GC | Cj0987 | R |
| hipOR565bam | 919168-919190 | CGC GGA TCC GCG TTC AAT ACT ATA ACT ATC CGA AG | hipO | R |
| hipOF574mfeb | 919131-919110 | AAC AAT TGT TAA GGC AAA AGA TCC TAT TTA TG | hipO | F |
| waaFpr1bamF | 1081867-1081844 | CGC GGA TCC GCG TCA TAG ATG AGA GTT TTT AAG TAA | waaV | F |
| waaFpr1bamR | 1080854-1080882 | CGC GGA TCC GCG TTC CTA AAT TTT GTT AAA ATA ATA AAA AC | gmhA | R |
| cj0984F681 | 918516-918534 | ACT CAA AGA AAT TCA AAT C | Cj0984 | F |
| pBSC103Fbam | NAc | CGC GGA TCC GCG ATC GTA TGG AGC GGA CAA CG | cat | F |
| waaFF1 | 1080908-1080930 | ATG AAA ATT TTT ATA CAT CTT CC | waaF | F |
| cj1449F23 | 1388207-1388232 | TTA GAA TTT ATA AAA AAT GAG CAG C | Cj1449 | F |
| kpsTR640 | 1385624-1385652 | TAT TCC TTC ATC TAC ATC ATC ATA AAC C | kpsT | R |
| kpsMF481bam | 1387224-1387201 | CGG GGA TCC CGA TTA TTT GGC ATT TTG TGG AAC C | kpsM | F |
| kpsMR320bam | 1387385-1387410 | CGG GAT CCC GTT CTA GCA ATA AAT ACA TGT ATA GG | kpsM | R |
| Cj0984F646 | 918842-918501 | AAA GAG CTT TTA GCA AAC C | Cj0984 | F |
| kpsMpr1bamR | 1387775-1387825 | CGG GAT CCC GAA CAA TGC TTT AGG ACT TAG | Cj1449 | R |
| kpsER120bam | 1386144-1386157 | CGG GAT CCC GTG GTG CTG CAA TC | kpsE | F |
| kpsMF1 | 1387679-1387699 | ATG TTA AAT GTA ATT TAT GC | kpsM | F |
a
Location based on the sequence of C. jejuni strain 11168.
b
Restriction sites underlined; BamHI (GGATCC) and MfeI (CAA TTG).
c
Not applicable.