Table 2.

Selected genes from virulence strain M. tuberculosis H37Rv and their primers used for complementation of M. tuberculosis H37Ra

Strain	Gene		Primer	Variation compared with H37Rv	Product
P10	pstA1	Rv0930	5′-atctctagatgtttaagcagggcaacgtgtcg-3′	R305* (C914T)	Phosphate ABC transporter, transmembrane protein
			5′-tattctagagctaccgttcaccggcctcaaca-3′
P19	lpdA	Rv3303c	5′-tacgtctagaatccgttgtggtgtccgaggaa-3′	promoter**	Flavoprotein disulfide reductase
			5′-cgaatctagaagcgtggtcagcgacagttca-3′
P17	pabB	Rv1005c	5′-tacgtctagaactggtgttggtgctggcgatag-3′	promoter (A-56T)	Aminodeoxychorismate synthase component I
			5′-cgaatctagacaggaccgcagccagccgat-3′
P15	mazG	Rv1021	5′-tacgtctagaccgaagaacccaaggatgtgcg-3′	A219E (C656A)	Nucleoside triphosphate pyrophosphohydrolase
			5′-cgaatctagagacacttgggattctcgacctcg-3′
P18	nrdH	Rv3053c	5′-tacgtctagaattgggtcagacgggtatgg-3′	promoter Δ-194—180GTGCCACCCCCTAG	Glutaredoxin electron transport protein NrdH
			5′-cgaatctagagcaatgacctgtttggggac-3′
P14	pknH	Rv1266c	5′-cgctctagacacattcgcatctgtgaccatgaa-3′	R607Q (G1820A)	Transmembrane serine/threonine-protein kinase H
			5′-tcgtctagagatgaagcccagaatcgctacgga-3′
P16	nadD	Rv2421c	5′-tacgtctagaatgtgtgtgcaagcagcttcgcc-3′	promotor (A-44C)	Nicotinic acid mononucleotide
			5′-cgaatctagagtaacccgcctgtcgcattttct-3′
P11	phoP	Rv0757	5′-agttctagacggcatcacccaacgcttgtttg-3′	S219L (C655T)	Two component system response regulator
			5′-aattctagagctgcgaccaggcgtacccgtag-3′
P12	marR	Rv0880	5′-ctgtctagaacctcgagcagcacgggtggtag-3′	R67K (G200A, G201A)	MarR family transcription regulator
			5′-gagtctagacggccagatcatccttgagctcg-3′
P20	ilvD	Rv0189c	5′-tacgtctagagcacgattacgctgcccctacc-3′	V285G (T851G)	Dihydroxy-acid dehydratase
			5′-cgaatctagagtggtcgcacatcatcgcaggt-3′
P13	luxR	Rv0890c	5′-agttctagatacagttgcgcgacgaactctatg-3′	P866A (C2596G)	LuxR family transcription regulator
			5′-atatctagatctccgggaccactgtcatacgag-3′
PolyG	Vector control				Control with empty vector

^**LpdA promoter GCTGCGGTGCGGGTCATCGTCAAGCTGTGCTT-154—123TGCGCGGCGTGCATCGTCGCCGAGCTGTGTTG.