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ExsC is not required for the expression of T3SS1 genes in the ΔexsD strain. (A) Protein samples isolated from ΔexsD exsC (lane 1) and ΔexsD (lane 2) strains grown in LB-S were probed with polyclonal antibody against Vp1656 (upper), Vp1659 (middle) and DnaK (lower); (B) RT-PCR analysis was used to detect mRNA transcripts from a subset of T3SS1 genes for the ΔexsD exsC (lane 1) and ΔexsD strains grown in LB-S.
