Figure 7.

The N-terminus of ExsC is required for ExsC to bind with ExsD. (A) Schematic representation of fusion proteins between full-length exsC and GST (upper), and between N-terminus truncated exsC and GST (lower). “Deletion” indicates the location of the 5-prime deletion for the truncated exsC sequence. (B) Whole-cell lysate and elution from NY-4:pGST-exsC-His pexsD-HA or from NY-4:pGST_exsC_Δ5′-His pexsD-HA strains probed with anti-His (upper) or anti-HA (lower) antibodies. The truncated ExsC_Δ5′protein does not bind ExsD.