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. 2010 Jul-Aug;1(4):260–272. doi: 10.4161/viru.1.4.12318

Figure 8.

Figure 8

Full-length ExsC is required for transcription and expression of T3SS1 genes. (A) Strains were cultured separately in LB-S and then cell lysates from NY-4:pGST_exsC-_Δ5′-His (lane 1) and NY-4:pGST-exsC-His (lane 2) were probed with anti-Vp1656, anti-Vp1659, anti-His and anti-DnaK; (B) RT-PCR analysis showing mRNA transcripts for a panel of T3SS1 genes for the NY-4:pGST-exsC-His and NY-4:pGST_exsC_Δ5′-His strains after growth in LB-S. SecY, a housekeeping gene, served as a positive control for the RT-PCR experiment.