Table 1.
Strains and plasmids used in this study
| Strains and plasmids | Descriptions | Sources |
| E. coli | ||
| S17-1λpir | thi pro hsdR hsdM+ recA RP4-2-Tc::Mu-Km::Tn7 λpir | 57 |
| S17_pDM4_exsC1 + 2 | S17 carrying pDM4_exsC1 + 2 | This study |
| S17_pMMB207_exsC | S17 carrying pMMB207_exsC | This study |
| S17_pMMB207_bla | S17 carrying pMMB207_bla | This study |
| S17_ pDM4_exsA_HA_Insertion | S17 carrying pDM4_exsA_HA_Insertion | This study |
| S17_ pDM31_Ptac_exsC | S17 carrying pDM31_Ptac_exsC_His | This study |
| S17_ pMMB207_exsD_HA | S17 carrying pMMB207_exsD_HA | This study |
| S17_ pMMB207_exsA_HA | S17 carrying pMMB207_exsA_HA | This study |
| S17_ pGST_exsC_His | S17 carrying pRY107_GST_exsC_His | This study |
| S17_ pGST_ exsC_Δ5′-His | S17 carrying pRY107_GST_exsC_Δ5′-His | This study |
| S17_ pDM4_exsA_HA_Insertion | S17 carrying pDM4_exsA_HA_Insertion | This study |
| V. parahaemolyticus | ||
| NY-4 | Clinical isolate O3:K6 | 47 |
| ΔexsC | exsC (vp1701) deletion mutant | This study |
| ΔexsD exsC | exsC and exsD double deletion mutant | This study |
| ΔexsD | exsD deletion mutant | 47 |
| ΔexsC_pMMB207_exsC | ΔexsC strain with pMMB207_exsC | This study |
| NY-4_pMMB207_exsC | NY-4 with pMMB207_ exsC | This study |
| ΔexsC_pMMB207_bla | ΔexsC strain with pMMB207_ bla | This study |
| NY-4_pMMB207_bla | NY-4 with pMMB207_ bla | This study |
| NY-4:pexsA | NY-4 strain with pMMB207_ exsA | 47 |
| NY-4:pexsD-HA pexsC-His | NY-4 with pDM31_Ptac_exsC_His and pMMB207_exsD_HA | This study |
| NY-4:pexsD-HA | NY-4 with pMMB207_exsD_HA | This study |
| NY-4:pexsD-HA-pexsA-His | NY-4 with pDM31_Ptac_exsA_His and pMMB207_exsD_HA | This study |
| NY-4:pGST-exsC-His pexsD-HA | NY-4 with pRY107_GST_exsC_His and pMMB207_exsD_HA | This study |
| NY-4:pGST-exsC-His pexsA-HA | NY-4 with pRY107_GST_exsC_His and pMMB207_exsA_HA | This study |
| NY-4:pGST_ exsC_Δ5′-His pexsD-HA | NY-4 with pRY107_GST_ exsC_Δ5′-His and pMMB207_exsD_HA | This study |
| NY-4:pGST-exsC(7-148)-His pexsD-HA | NY-4 with pRY107_GST_exsC(7-148)-His and pMMB207_exsD_HA | This study |
| NY-4:pGST-exsC(13-148)-His pexsD-HA | NY-4 with pRY107_GST_exsC(13-148)-His and pMMB207_exsD_HA | This study |
| NY-4:pGST-exsC(27-148)-His pexsD-HA | NY-4 with pRY107_GST_exsC(27-148)-His and pMMB207_exsD_HA | This study |
| NY-4_pDM4_exsA_HA_Insertion | NY-4 with HA tagged exsA in the chromosome | This study |
| ΔexsC_pDM4_exsA_HA_Insertion | ΔexsC with HA tagged exsA in the chromosome | This study |
| Plasmids | ||
| pDM4 | A suicide vector with ori R6K sacB; Cmr | 58 |
| pMMB207 | RSF1010 derivative, IncQ lacIq Cmr Ptac oriT | 59 |
| pDM31 | TcR; p15A origin, pACYC184 derivative that lacks cmr and includes mob | 54 |
| pRY107 | KmR, pWSK29 MCS oriT mob+ | 56 |
| pDM4_exsC1 + 2 | Flanking region sequences of exsC cloned into pDM4 | This study |
| pMMB207_exsC | exsC coding sequences with sequences for 6 His amino acids at the C-terminus cloned into pMMB207 | This study |
| pMMB207_bla | Partial blaTEM-1 cloned into pMMB207 | This study |
| pDM31_Ptac_exsC_His | exsC coding sequence with sequence for 6 His amino acids at the C-terminus and Ptac promoter sequence at the N-terminus cloned into pDM31 | This study |
| pMMB207_exsD_HA | exsD sequence with HA sequence at the C-terminus cloned into pMMB207 | This study |
| pMMB207_exsA_HA | exsA sequence with HA sequence at the C-terminus cloned into pMMB207 | This study |
| pDM31_Ptac_exsA_His | exsA sequence with sequence for 6 His amino acids at the C-terminus and Ptac promoter sequence at the N-terminus cloned into pDM31 | This study |
| pRY107_GST_exsC_His | exsC sequence with GST sequence at the N-terminus and 6 His sequence at the C-terminus cloned into pRY107 | This study |
| pRY107_GST_ exsC_Δ5′-His | N terminal 102 bp truncated exsC sequence with GST sequence at the N-terminus and 6 His sequence at the C-terminus cloned into pRY107 | This study |
| pRY107_GST_exsC(7-148)-His | N terminal 18 bp truncated exsC sequence with GST sequence at the N-terminus and 6 His sequence at the C-terminus cloned into pRY107 | This study |
| pRY107_GST_exsC(13-148)-His | N terminal 36 bp truncated exsC sequence with GST sequence at the N-terminus and 6 His sequence at the C-terminus cloned into pRY107 | This study |
| pRY107_GST_exsC(27-148)-His | N terminal 78 bp truncated exsC sequences with GST sequence at the N-terminus and 6 His sequence at the C-terminus cloned into pRY107 | This study |
| pDM4_exsA_HA_Insertion | exsA with HA sequence at the C-terminus cloned into pDM4 | This study |