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. Author manuscript; available in PMC: 2012 Mar 15.
Published in final edited form as: Chem Biol Interact. 2011 Jan 15;190(1):62–72. doi: 10.1016/j.cbi.2011.01.009

Figure 2. ‘Stemness’ marker mRNA expression is significantly decreased in hepatic-like cells.

Figure 2

Passage-matched HESCs were cultured in parallel on either hFF feeder layers in hESC media (hESCs exp) or on collagen in hepatocyte media (Hepatic-like cells). After 10 days in culture cells were harvested and RNA was isolated, converted to cDNA, subjected to real-time PCR, and the data analyzed using the ΔΔCT method to determine fold expression levels of NANOG and alkaline phosphatase relative to low-passage hESCs. Control data (left graph) depict means of expression levels of replicate determinations of a typical low-passage culture of hESCs (hESCs control), in contrast to mean expression levels of replicate determinations performed using a pooled sample of six adult primary human hepatocyte donors (Hepatocytes). In these control experiments, the replicate values were reproducible within a ≤ 5% error range. Experimental data (right graph) depict expression levels of hESCs (hESCs exp) and passage-matched hESCs subject to our hepatic differentiation protocol (Hepatic-like cells). Experimental data are expressed as mean +/− standard deviations of at least two independent trials using hESCs from different passages. * p < 0.05; ** p < 0.01.